Reporter
Part:BBa_K325210:Design
Designed by: Hannah Copley and Theo Sanderson Group: iGEM10_Cambridge (2010-09-15)
Red Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2060
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 951
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 900
Illegal AgeI site found at 2602 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 332
Illegal SapI.rc site found at 2116
Design Notes
Codon optimisation, making compatible with BioBrick assembly format.
Source
This was codon-optimised for E.coli and syntesised by DNA 2.0.