Reporter

Part:BBa_K325210:Design

Designed by: Hannah Copley and Theo Sanderson   Group: iGEM10_Cambridge   (2010-09-15)


Red Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 900
    Illegal AgeI site found at 2602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 332
    Illegal SapI.rc site found at 2116


Design Notes

Codon optimisation, making compatible with BioBrick assembly format.


Source

This was codon-optimised for E.coli and syntesised by DNA 2.0.

References